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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Direct Reprogramming of Spiral Ganglion Non-neuronal Cells into Neurons: Toward Ameliorating Sensorineural Hearing Loss by Gene Therapy
doi: 10.3389/fcell.2018.00016
Figure Lengend Snippet: Validation of RNA-seq by droplet digital PCR, and gene expression profiling of neuron-/Schwann cell-/fibroblast-specific genes. (A) Heatmap showing Pearson correlations among samples in terms of the common logarithmic counts of genes with non-zero counts. Samples in each group correlated well with each other and the largest difference was between PAN and VC. (B) Principal component analysis (PCA). The regularized logarithmic transformation was applied to the normalized counts and PCA was performed on the top 500 most variable genes across samples. Three samples in each group were well clustered. (C,D) Validation of RNA-seq data using droplet digital PCR (ddPCR). Relative value of each group compared to iN (= 1.00) is shown. (C) Relative value of TPM was calculated for known neuron markers (TuJ1 and Prph), glial marker (Sox2), and fibroblast marker (Tnc). (D) Relative value of ddPCR expression data for the same 4 genes validated our RNA-seq data. Error bars represent standard error of the mean (SEM). (E) A heatmap for reportedly specific genes for pan-neuronal, synaptic, ion-channel, transcription factors, cytoskeletal reorganization, oligodendrocytes, Schwann cells, mesenchymal cells and mitosis. Red-white-blue spectrum indicates high-moderate-low expression level for each gene.
Article Snippet: In contrast, we found the
Techniques: Biomarker Discovery, RNA Sequencing, Digital PCR, Gene Expression, Transformation Assay, Marker, Expressing